Serial Dilution Calculator

Calculate dilution schemes for IC50 assays, dose-response curves, and antibody titrations

Dilution Parameters

Starting Concentration

Final Concentration

Number of Dilution Points

Volume per Well/Tube (μL)

Dilution Factor (Optional)

Leave blank for logarithmic series (recommended for IC50)

Enter parameters and calculate to see dilution scheme

IC50 Dose Response

• 10 μM → 1 nM

• 8-10 points

• Logarithmic spacing

Antibody Titration

• 1:50 → 1:12,800

• 2-fold serial dilutions

• 8 points

Standard Curve

• 1000 ng/mL → 1 ng/mL

• 10-fold dilutions

• 4-6 points